Salmonella spp. Assay Validation

Internal AOAC-style validation results for the SpectacularOne Salmonella spp. Detection System. Built for enterprise scale. Backed by lab-grade performance.

Notice

The SpectacularOne system is currently undergoing AOAC PTM validation (Est. Q1 2026).

The results shown below are from internal AOAC-style performance studies conducted to showcase how our system was evaluated.

Once peer-reviewed publications become available, they will be displayed here.

Overview

The SpectacularOne Salmonella spp. Detection System was evaluated using internal AOAC-style validation protocols across inclusivity, exclusivity, limit of detection (LOD), and fractional recovery in real food matrices.

This page summarizes our internal showcase study, which included performance across 20 Salmonella spp. serovars, cross-reactivity controls, and spiked sample recovery experiments.

Executive Summary

  • Inclusivity: 20/20 Salmonella spp. serovars detected (100%)
  • Limit of Detection (LOD): ~11,000 CFU/ml (S. Derby)

Validation Figures

Positive vs Negative Controls (Exclusivity Check)

This figure contrasts amplification signals between known positive Salmonella spp. samples and non-Salmonella spp. controls. All non-Salmonella spp. controls show no signal, confirming 100% assay exclusivity.

Controls verification

Detection by Serotype

The bar chart shows individual amplification results for 20 unique Salmonella spp. serovars. Every serotype generated a valid detection signal, reinforcing 100% inclusivity performance.

Serotype detection chart

Conclusion

The SpectacularOne system demonstrates internal AOAC-style performance with 100% inclusivity, 100% exclusivity, and consistent LOD across replicates.

To receive the full technical documentation, including method development and amplification conditions, contact our biology team directly.